Biochemical characterization of serratia liquefaciens and. The taxonomy of the other currently recognized serratia species is clearer. Serratia is a genus of gramnegative, facultatively anaerobic, rodshaped bacteria of the family. Serretia rubidaea for biofilm development capability jabun naher1, shahidul a. Carotenoids are effective antioxidants and are widely used as food colorants. Biblioteca italiana o sia giornale di letteratura, scienze e. Only serratia marcescens has been established as a. Files are available under licenses specified on their description page. Serratia are widespread in the environment, but are not a common component of the human fecal flora.
Sustainable biosurfactant produced by serratia marcescens. Analysis of the genomic sequences and metabolites of serratia. Serratia rubidaea synonyms, serratia rubidaea pronunciation, serratia rubidaea translation, english dictionary definition of serratia rubidaea. This species was first described as bacterium rubidaea in 1940 and presently recognized as s. Serratia liquefaciens bloodstream infections from contamination of epoetin alfa at a hemodialysis center. It is capable of growth between 4oc and 37oc with some isolates capable of growth at 40oc.
The isolate was belonged to the genus serretia and provisionally identified as. Serratia is a short, facultatively anaerobic, gramnegative, rodshaped bacteria of the enterobacteriaceae family. Maldi biotyper smart ca system the highend option with increased sample throughput the maldi biotyper ca system is brukers pioneering solution for rapid and accurate microbial. A total of 167serratia strains was isolated from these plant samples and identified to species and biogroups. Pdf isolation and characterization of serratia rubidaea. Serratia rubidaea definition of serratia rubidaea by. Using serratia plymuthica to control fungal pathogens of plants. If the species was not widely studied, the american type culture collection atcc was used to determine the optimal ph for storage. Choose from 80 different sets of serratia flashcards on quizlet. Using serratia plymuthica to control fungal pathogens of. Aug 15, 2019 biochemical test and identification of serratia marcescens. Pursuant to article 16 of annex ii to directive 199931ec.
Serratia species, serratia marcescens, serratia liquefaciens, serratia. The most common and pathogenic of the species in the genus, s. The infection was cleared after adequate treatment with antibiotics. All structured data from the file and property namespaces is available under the creative commons cc0 license. Outbreaks of serratia marcescens and serratia rubidaea bacteremia. In 1978, serratia odorifera wasdescribedasanewspecies of. Nowadays, recognized species within the genus serratia are s.
Escherichia, klebsiella, enterobacter, citrobacter, serratia presence of coliforms in water is indicate of impure water and of poor sewage treatment i. This study was performed to compare the growth dynamics of serretia in single and coculture biofilms condition and also evaluated its antagonistic effect against pathogenic bacteria. Mar 19, 2018 this page was last edited on 19 march 2018, at. One preserved isolated subculture was identified on the basis of their morphological, cultural and biochemical characteristics. The full text of this article is available as a pdf 157k. Serratia rubidaea definition of serratia rubidaea by the. Pdf isolation and characterization of serratia rubidaea from dark. A serratia rubidaea isolate with wetting activity when grown at 30 but not 37 degrees c was examined for the production of specific lipids. Proizvodnja lipaze s pomocu bakterije serratia rubidaea. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin installed and enabled in your browser. Taxonomically, the genus serratia is confusing, and currently there are 14 recognized species, with 2 subspecies, in the genus table 1. Biochemical characteristics, antimicrobial sensitivity and clinical significance sd puthucheary mbbs, dipbactc and yf ngeow mbbs, mscc summary serratia marcescens has become an important nosocomial pathogen. Interest in biological control of plant pathogens has increased in recent years fuelled by trends in agriculture. Abstract microorganisms are known to produce a variety of pigments.
Isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits. Serratia rubidaea as an invasive pathogen article pdf available in journal of clinical microbiology 341. They have created significant clinical challenges for clinicians because they are not consistently identified by routine screening methods and are. Biochemical test and identification of serratia marcescens. Studies on the response of microbes to environmental stressors form a thrust area of contemporary. Lettera di bartolomeo bizio al chiarissimo canonico angelo bellani sopra il fenomeno della polenta porporina. Five isolates showing spots on tomato fruits in the pathogenicity test with healthy tomato fruits belong to the. The only serratia species recognized in the 8th edition of bergeys manual was serratia marcescens 1. Effects of bacterial growth and gas phase ammonia on medium ph and serratia rubidaea growth. Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent.
Serratia marcescens after initial outbreaks in the northeastern u. Natural antimicrobial susceptibilities of strains of unusual serratia. Isolation of serratia plymuthica from a human burn site. Studies on the antagonistic effect of serretia rubidaea. Introduction the genus serratia rubidaea is gramnegative speculator pathogens, known to survive harsh environmental surroundings and is a leading cause of hospital acquired infections. Nag agar with phenol red was used to visualize ph changes. Members of the genus serratia, particularly the type species serratia marcescens, cause important infections in humans, animals, and insects. Ammonia produced by bacterial colonies promotes growth of. Using serratia plymuthica to control fungal pathogens of plants core.
Request pdf serratia rubidaea bacteremia serratia rubidaea is an opportunist. In this study, surface tension was used as response variable, and the effects of independent variables lactose, cww and co concentrations as well as the interactions of them, were analyzed by estimated effects represented in table 3 and fig. Uniform and characteristicserratia populations were found in figs and coconuts. Investigation of lipase production by milk isolate serratia rubidaea. The observed ph and temperature range optimum for maximum lipase production were 78 and 3040 c, respectively. Serratia rubidaea motile, red pigment enterobacter aerogenes motile, no pigment. It was discovered in 1819 by bartolomeo bizio in padua, italy. During a period of seven years 123 strains, both pigmented and nonpigmented varieties were isolated. Production of extracellular lipase in submerged culture of serratia rubidaea has been investigated. Serratia rubidaea is an opportunist pathogenic bacterium, it is rarely identified in man. The clinical significance of the organism was undetermined due to its apparent eradication from this location by therapy with topical 1% silver sulfadiazine.
Pdf investigation of lipase production by milk isolate. Page designed through the cooperative efforts of interagency itis teams. They appear ubiquitously inon soil, water, plants and animals, including humans. However, symptoms depend on the infection site and disease. When it enters the bloodstream, endotoxins are released that cause fever, septic shock, thrombocytopenia and disseminated intravascular coagulation. Pdf serratias are widely distributed in soil, air and water, plantand animalassociated bacteria. Two novel lipids rubiwettins r1 and rg1 were isolated and shown to be able to lower the surface tension of saline to 26 mnm. Serratia species, serratia marcescens, serratia liquefaciens, serratia plymuthica, serratia rubidaea, serratia fonticola, serratia marnorubra, serratia proteamaculans, and serratia odorifera, are opportunistic pathogens and can be isolated from water, soil, plants, and air. Investigation of lipase production by milk isolate. Supporting information the short term effect of the soil. Jun 20, 2016 the observed increase in plant growth by acc deaminaseproducing serratia marcecens cdp is also supported by other observations, where acc deaminaseproducing bacteria were shown to enhance plant growth by lowering the ethylene level 58, 59. Based on 16s rrna gene sequence analysis, the isolate was identified as serratia marcescens.
Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. Isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits article pdf available in phytoparasitica 392. The genus serratia is composed of facultative anaerobic, rodshaped gammaproteobacteria with peritrichous flagella summarized in mahlen, 2011. Serratia species secrete several virulence factors, such as dnase, lipase, gelatinase, hemolysin, proteases, chitinase.
Bacterial contamination of fresh tomato fruits is of great concern. Apart from serratia marcescens and the serratia liquefaciens complex s. Serratia rubidaea is a less welldescribed member of the genus and is chiefly found in soil, water and food. The lipase production was optimized in shake flask experiments. Sustainable biosurfactant produced by serratia marcescens ucp. Oxidase negative gram negative rods enterobacteriaece. Voluntary surveillance of bacteraemia klebsiella spp. Alternatively, you can download the file locally and open with any standalone pdf reader. Voluntary surveillance of bacteraemia due to klebsiella spp. Microbial pigments find several applications and are thus of commercial importance. From naturally infected tomato fruits showing dark brown irregularly shaped spots, 36 bacterial isolates were recovered and identified on phenotypic characteristics and sequences of the gene encoding the 16s rrna. Serratia marcescens is the primary pathogenic species of serratia.
Mar 08, 2011 isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits abdalla, mohamed. Paraplegia could be a predisposing factor for this organism to cause urinary tract infection. Hosted by the usgs core science analytics and synthesis. Biblioteca italiana o sia giornale di letteratura, scienze e arti anno viii 30. Serratia rubidaea bacteremia request pdf researchgate. Genero enterobacter y serratia infeccion salud publica. Although several recent studies disclosed a number of interesting biological properties of prodigiosin, this paper reports a higher value of prodigiosin production by a newly isolated indigenous marine bacteria serratia rubidaea rather than serratia marcescens from a relatively cheap medium. Serratia species are opportunistic gramnegative bacteria classified in the tribe klebsielleae and the large family enterobacteriaceae. Flow chart page 4 flow chart gram negative reaction. For example, patients with serratia sepsis may present with fever, chills, shock, and respiratory. Serratia rubidaea an aerobic gramnegative bacillus that grows readily on most microbiological media producing a red prodigiosin colony. Highest tensioactive activity detected in current study in flasks with agitation can be linked to the physiological function of the biosurfactants. If the species was not widely studied, the american type culture collection atcc was used to.
Brenner established serratia rubidaea as a later homotypic synonym of serratia marinorubra zobell and upham 1944 approved lists 1980 in 1984. Part of the microgen bioproducts ltd, unit 1, watchmoor point, camberley, surrey gu15 3ad, united kingdom t. Serratia odorifera biogroup causing invasive infection. Isolation and characterization of serratia rubidaea from dark. Antagonistic activity of bacillus amyloliquefaciens subsp. A study was made of the biochemical reactions given by 109 cultures of enterobacter liquefaciens and 49 strains of serratia rubidaea.
In their paper about the taxonomy of serratia, grimont and coworkers 2 described four species within the genus serratia. Voc emission of various serratia species and isolates and. Among the pgp traits, the isolate was found to be positive for. Urinary tract infections are asymptomatic for 3050% of infected individuals, with the rest.
The present study demonstrates the plant growth promoting pgp potential of a bacterial isolate cdp isolated from capparis decidua plant, and its ability to protect plants from the deleterious effect of biotic and abiotic stressors. S7 1 ec council decision of 19 december 2002, establishing criteria and procedures for the acceptance of waste at landfills. Serratia rubidaea is an opportunist pathogenic bacterium, it is rarely identified in man, and when so, generally found in the respiratory tract, wounds, feces, bile, but also in blood. The full text of this article is available in pdf format. Isolation and characterization of serratia rubidaea from. Serratia serashea, a genus of motile, peritrichous. They are gram ve, catalase positive, oxidase negative, mr negative and vp positive bacteria. Klebsiella pneumoniae, enterobacter aerogenes, serratia marcescens, and serratia rubidaea produced a positive reaction in 4 h. Theorganism is rarely reported to occur in clinical specimens.
Serratia is a genus of gramnegative, facultatively anaerobic, rodshaped bacteria of the family yersiniaceae. The saprophytic bacterium serratia plymuthica was recovered from a facial wound burn site of a pediatric patient. Use of bileesculin agar for rapid differentiation of. Serratia species isolated from plants springerlink. Majority of microbes reported produce carotenoids belonging to myxococcus browning et al. The use of this medium in conjunction with triple sugar ironlysine iron agar has been of great value in differentiating the klebsiellaenterobacter serratia. The species serratia rubidaea was originally described by ewing et al. Using the selective caprylatethallous agar medium, the presence ofserratia species was systematically examined in 623 plant samples. The other remaining eight genera exhibited varying results. Page 4 flow chart gram negative reaction possibilities eliminated pseudomonas enterobacteriaceae neisseria veillonella bacillus mycobacterium lactobacillus, listeria kurthia micrococcus, planococcus, sporosarcina staphylococcus streptococcus enterococcus morphology rods possibilities eliminated pseudomonas enterobacteriaceae neisseria veillonella. Surfaceactive novel glycolipid and linked 3hydroxy fatty. Serratia is a ubiquitous opportunistic pathogen that is frequently present under damp conditions in food, plants, animals, soil, and household items e.
283 1009 983 1331 243 964 1454 371 594 1625 51 1043 812 1379 1019 1515 458 944 1141 218 291 1509 838 1070 118 119 319 416 256 580 72 1227 340 172 1344 1213 1380 766